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The relative expression level was calculated as a ratio of the mRNA level to the transcription level at 0 d after ACC treatment.
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The standard curve for each primer set was prepared by plotting serial cDNA dilutions to 5 against the threshold cycle CT. Displacement experiments indicate that ACC binds with a 5. Tip 2: Girls' Responses. Accumulation and transport of 1-aminocyclopropanecarboxylic acid ACC in plants: Sex dating Uji dating Uji status, considerations for future research and agronomic applications.
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